By car:
From the A road SS16: at Monopoli take the
provincial road toward Alberobello.
from the Bologna - Taranto motorway exit at Gioa del Colle
in the direction of Alberobello

By Bus:
The company is Marozzi
more detailed information can be found on their web site
http://www.marozzivt.it/HomePage.htm

By Train:
The line needed is south east, the jorney from Bari to Alberobello takes between around 90 mins. more detailed information can be found at:
http://www.fseonline.it/fse/italiano.htm